The Predicted Mannosyltransferase GT69-2 Antagonizes RFW-1 To Regulate Cell Fusion in Neurospora crassa
نویسندگان
چکیده
منابع مشابه
Cell Fusion in Neurospora crassa
The primary research aims of this thesis were the identification of novel cell fusion mutants of Neurospora crassa and the subsequent functional characterization of selected candidate proteins during conidial anastomosis tube (CAT)-mediated cell fusion by means of genetic, molecular, biochemical and live-cell imaging analysis. Chapter 1 provides a general introduction of the model organism and ...
متن کاملThe ham-5, rcm-1 and rco-1 genes regulate hyphal fusion in Neurospora crassa
Mutants of Neurospora crassa unable to participate in vegetative hyphal fusion (anastomosis) were isolated and characterized. From this analysis, three genes, rcm-1, rco-1 and ham-5, were identified and shown to be required for hyphal fusion. The rcm-1 and rco-1 genes are homologues of the Saccharomyces cerevisiae SSN6 and TUP1 genes, which encode a dimeric transcription factor in yeast. We dem...
متن کاملCell fusion in the filamentous fungus, Neurospora crassa.
Hyphal fusion occurs at different stages in the vegetative and sexual life cycle of filamentous fungi. Similar to cell fusion in other organisms, the process of hyphal fusion requires cell recognition, adhesion, and membrane merger. Analysis of the hyphal fusion process in the model organism Neurospora crassa using fluorescence and live cell imaging as well as cell and molecular biological tech...
متن کاملIdentification and characterization of LFD-2, a predicted fringe protein required for membrane integrity during cell fusion in neurospora crassa.
The molecular mechanisms of membrane merger during somatic cell fusion in eukaryotic species are poorly understood. In the filamentous fungus Neurospora crassa, somatic cell fusion occurs between genetically identical germinated asexual spores (germlings) and between hyphae to form the interconnected network characteristic of a filamentous fungal colony. In N. crassa, two proteins have been ide...
متن کاملLive-cell imaging of vegetative hyphal fusion in Neurospora crassa.
The process of hyphal fusion (anastomosis) in growing colonies of Neurospora crassa, stained with the membrane-selective dyes FM1-43 and FM4-64, was visualized by confocal microscopy. Time-lapse, live-cell imaging illustrated the dynamics of hyphal growth and anastomosis during its pre-contact, contact and post-contact, and post-fusion stages. Fusion-competent hyphae were morphologically distin...
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ژورنال
عنوان ژورنال: mBio
سال: 2021
ISSN: 2150-7511
DOI: 10.1128/mbio.00307-21